About NeoTREX Cancer Research Supercluster Disease Areas Cancer in Pet Animals Personnel COO - Opgenorth CSO - Withrow Dir. of Partnership Dev. - Hardy Dir. of Business Dev. - Foster General Inquiry Contact Information Advisory Colorado Bioscience Colorado Bioscience Association Colorado Bioscience Roadmap 2008 Downloadable Documents Cancer Supercluster RFP documents NeoTREX Brochure Cancer Supercluster Featured Research Facilities and Resources Partnering Featured Research Cancer Biology Clinical Trials Musculoskeletal Oncology Therapeutics Resources Animal Models Cytometry and Clinical Immunology Facilities Animal Cancer Center Ann Avery Lab Grant Funding Imaging Equipment Laboratories and Specialized Equipment Pharmacology Radiation Therapy Tissue Archiving Start-ups Intellectual Property For Researchers Supercluster Activities Cluster Leaders Technology Transfer Process Legal Agreements CDA's Consulting IDF Contact Us Email Registration General Inquiry

ACC Laboratory Equipment             Common Equipment Milliq water system Typhoon ** Micro/standard top-loading balance Perkin Elmer g Counter BioRad Gel Doc Image Eraser (Autoradiography) Still ABI Prism - Real-time PCR ** ThermoHybaid PCR BioRad Spectrophotometer 3000 Thermo Hybaid Thermocyclers LN2 Storage Autoclave **     Pharmacology Lab MDS Sciex 3200 Q-TRAP triple quad mass spec-ACC 263 Turbo IonSpray Source Agilent 1200 Series Binary Pump HPLC system HTC Pal LEAP Autosampler Parker Balston Source 5000 N2 generator Xenogen Imager ** Savant Speed Vac Systems (2) Sonic Dismembrator Anesthesia Machine Mouse Metabolic Cages (4) Microcentrifuge Shaking water bath Analytical Scales (2) pH Meter Vial crusher     Molecular Pathology Ventana Medical Systems Discovery XT automated stainer Carl Zeiss Axioplan 2 imaging scope AxioCam HRc Carl Zeiss camera Axio Vision 4.3 software from Carl Zeiss     Tumor Immunology Dako Flow Cytometer Nexelcom Cell Counter High-speed centrifuge Virtis Lyophilizer Leica Cryostat Sonicator Perkin-Elmer g Counter         ACC Laboratory Skills      Cancer Genetics - Molecular PCR/Real Time PCR DNA analysis/sequencing Gene Chip Arrays Restriction Digests Cloning/subcloning PCR based generation of mutant constructs Western Blots/Northern Blots/Southern Blots Immunoprecipitation Licensed to handle radioactive materials      Cancer Genetics - Protein-to-Protein Immunoprecipitation GST - pulldown Biacore Yeast two-hybrid screening In vitro transcription/translation for protein expression/labeling Tissue Culture Skills: Transient and stable cell transfection Creating clonal isolates IHC MTT Scratch Tests (Cellular Assays)      Molecular Pathology PCR/RT-PCR RNA isolation cDNA synthesis IHC/IF Western Blots/Dot Blots Necropsy/Histology Image Analysis Lab animal care ISH/FISH - set up, but never done      Investigational Therapeutics Tissue culture - primary and established mammalian lines Bacterial culture Migration/Invasion Assays Flow cytometry Growth inhibition assays Anchorage - independent assays ELISAs - commercial and "home-grown" development Cryosectioning IHC/IFC PBMC/BMMC isolation Cryopreservation Western Blots RT-PCR/qRT-PCR LBT/MLR assays         Medical Oncology Thermo-Spectronic Biomate 3 spectrophotometer Labsystems Multiskan MCC340 plate reader (absorbance) Sratagene 2400 Stratalinker Fisher mini- and maxi-gel boxes Invitrogen Powerease 500 power supply Invitrogen Vertical Gel system     Cancer Genetics Inverted fluorescent microscope Gradient PCR machine Life Technologies Model 4001P/BioRad Power Pak 1000 3' Level II BioSafety hood dedicated to PCR     Investigational Therapeutics Refrigerated high-speed centrifuge, swing-out rotor/microfuge rotor LN2 storage system Electronic scale, Analytical balance BioTekT Plate Reader - luminescence, fluorescence, absorbance Inverted phase microscope Binocular light microscope Electroporater Stratagene Mx3000P® real-time PCR NovexT Gel apparatus Tissue "Tearor"T Life Technologies Model 4001P - programmable power supply Dry heat blocks (2) Rocker platform Microfuge (2) pH Meter      Musculoskeletal Lab Surgery table/anesthesia machine Xenogen imaging system ** Polytron Microfuge Refrigerated tabletop centrifuge Rocker platform UV-Vis spectrophotometer (temporary) Inverted phase microscope pH Meter LN2 storage system Sonicating water bath                Cancer Genetics - Interactions Electrophoretic Mobility Shift Analysis South-Western Blot Analysis Yeast One-hybrid screening Luciferase Promoter Assays Protein Expression and Isolation from Bacterial cultures Inducible & constitutive gene expression in mammalian systems      Pharmacology Lab Mammalian tissue culture Proliferation assays Death/apoptosis assays Immunocytochemistry/IHC Light/flourescent microscopy Flow cytometry Western blotting Animal handling, surgery, anesthesia Plasma and tissue collection HPLC/mass spectrometry Biological sample extraction/ preparation pharmacokinetic modeling Biochemistry gods      Tumor Immunology Immunostaining Multi-color Flow Cytometry Basic immunological assays Flow cytometry - assays; instrument maintenance/troubleshooting Tissue Culture ELISA PCR/Cloning IHC/IFA Protein Purification Antibody/Protein labeling Mouse wrangling - iv injection, grooming for tumor injections, surgical      Musculoskeletal Lab Xenogen imaging in vivo/in vitro ** Tissue culture Bacterial culture ELISA Animal surgery, anesthesia IHC Western Blots Xenograft models Cryosectioning Licensed to handle radioactive materials Plasma and tissue collection PBMC isolation Light/flourescent microscopy Necropsy Animal handling (IV, IP, SC, tumor injections)       ** included/proposed to be included in cost center (21 account)

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